Fluorogenic Immuno-Sensor Using Inverse Opal Hydrogel with Target Specific Aptamer Modification

Over 10million people have disabilities due to nervous system disorders. Alzheimer's, a representative degenerative brain disease, is characterized by irreversible cognitive dysfunction and occurs when amyloid plaque accumulates even before symptoms occur. Therefore, it is more important to prevent it through early diagnosis than to treat the disease. One of the early diagnosis system is Liquid biopsy that diagnoses using blood, urine, spinal fluid, etc. The aqueous humor is body fluid which fills the space between the lens and the cornea of the eye. The aqueous humor contains biomolecules come from central nervous system. Diagnosis platform using aqueous humor can be a promising platform for early diagnosis of neurodegenerative diseases.<br/><br/>Here, we made fluorescence aptamer conjugated inverse opal hydrogel lens (FAIOL). Intraocular lens are implanted for vision correction or cataract surgery at eye chamber which is filled with aqueous humor. FAIOL is aptamer conjugated intraocular lens system. When the biomarker approaches at FAIOL, quencher modified aptamer combines biomarkers and dissociates from fluorescence modified complimentary DNA. To raise specificity of FAIOL, AFIOL is made by MMA (methyl methacrylate) which is well known hydrophobic monomer. And for sensitivity of FAIOL, we applied inverse opal system that makes fluorescence signal amplification by photonic band gap. FAIOL can be a target independent sensor such as protein sensor by, DNA sensor, or RNA sensor. Early diagnosis of various neurological diseases will be possible through FAIOL