Subcutaneous Implantation of Amniotic Products in Sprague Dawley Rats: Artacent Wound and Artacent AC

Introduction:<br/>Although there are numerous placental membrane products currently being used in wound healing applications, it is not yet well-understood how these products interract with the wound bed as the wound heals. In this study, we assess subcutaneous implantation of varying amniotic membranes to better understand how these products support the wound healing process.<br/><br/>Methods:<br/>Four incisions were made on the back of each rat—two incisions left of the spine and two incisions to the right with each incision measuring approximately 2 cm. Four individual pouches were created by bluntly dissecting laterally at each incision site with scissors. A 1 x 1 cm implant was placed in each implantation site and samples were randomized in location. Implants were fixed in neutral buffered solution (NBS). Samples were stained with H&E and stained cross sections were evaluated for surface re-epithelialization, cellular infiltration, and remodeling.<br/><br/>Results:<br/>Representative histological images from each test group.<br/><br/>Discussion:<br/>In both Artacent Wound and Artacent AC, tissue ingrowth within the placental membrane product can be observed. Results support that the biological membranes provide an extracellular matrix that provides a natural scaffolding which supports the wound healing process.<br/><br/>Conclusion:<br/>In both Artacent Wound and Artacent AC, tissue ingrowth within the placental membrane product can be observed. Results support that the biological membranes provide an extracellular matrix that provides a natural scaffolding which supports the wound healing process.