Study of The Senescence Progress of Human Skin Fibroblast on Silica Nanobeads Array

When and Where

Nov 29, 2023
8:00pm - 10:00pm

Hynes, Level 1, Hall A

Presenter

Yerin Choi

Soojin Roh

Jin Seok Lee

Co-Author(s)

Yerin Choi¹,Soojin Roh¹,Jin Seok Lee¹

Hanyang University¹

Abstract

Yerin Choi¹,Soojin Roh¹,Jin Seok Lee¹

Hanyang University¹

Senescence is a physiological process that progresses with age. Cellular senescence is induced by stimuli such as telomere shortening and oxidative stress. In particular, it refers to the cell cycle arrest state. Senescence cells are characterized by reduced cell proliferation, migration, and speed. In addition, aging proteins p16, p21, and p53 are expressed.<br/>We study the adhesion changes of senescence cells on silica nanobeads (SiNBs) of various sizes. In particular, it analyzes how senescence changes due to different adhesion. We induced aging by adding Paclitaxel (PTX) to WS1 cells, which are human skin fibroblasts. PTX causes cellular senescence due to damage from ROS. The optimal PTX concentration was fixed, and the cell survival rate was confirmed by MTT assay. In particular, we have identified different adhesion on SiNB arrays of various sizes. In addition, changes in cell proliferation, migration, speed, and aging proteins were analyzed. As a result, we observed the correlation between the topographic factors of SiNB arrays and cellular senescence.

Keywords

biomaterial | Si

Symposium Organizers

Guillermo Ameer, Northwestern University

Gulden Camci-Unal, University of Massachusetts Lowell

Melissa Grunlan, Texas A&M University

Carolyn Schutt Ibsen, Oregon Health and Science University

Symposium Support

Silver
Acuitive Technologies, Inc.

Bronze
Center for Advanced Regenerative Engineering, Northwestern University
Nature Materials | Springer Nature