Pradakshina Sharma1
Jamia Hamdard1
A crucial aspect for managing epidemics efficiently, tailoring medical treatments to individuals, and developing novel drugs that are responsive to virologic response dependent dose, is the availability of cost-effective and user-friendly detection platforms suitable for point-of-care and home applications, among various other essential requirements. By combining the precision of 3D printing technology with electrochemical biosensors, this research introducing a new era in diagnostic capabilities. Amidst global health challenges exemplified by the formidable Chikungunya virus (CHIKV), the demand for rapid, accessible, and precise diagnostics has reached unprecedented heights. A 3D printed electrode cassette, meticulously tailored through Fused Deposition Modelling (FDM) 3D printing, maintains the integrity of the biosensor against an unpredictable external environment and alignment of the electrodes. These pioneering cassettes, combined with paper-based electrodes and the strategic integration of silver nanoparticles, amplify detection sensitivity. Employing cyclic voltammetry technique, the paper-based electrodes within these cassettes offer a profound understanding of electrochemical dynamics, enhancing the array of diagnostic tools. Remarkably, this biosensor has a detection range of 0.1ng/mL to 1µg/mL, all achieved within a response time of 20 seconds. Furthermore, its longevity, with a shelf life extending to 34 days, underscores its reliability even under prolonged usage. Moreover, the suitability and effectiveness of the newly devised 3D incorporated aptasensor were verified by presenting the Chikungunya virus antigen into blood-serum samples. This marks a new era where cutting-edge biosensing technologies have the potential to transform healthcare by delivering high-quality diagnostics that are both economically feasible and globally available.