Exploring Methods to Control Actin Dynamics with Implications in Synapse Formation

This presentation will describe the development of small molecule tools that can directly modulate the bundling of neuronal actin filaments <i>in vitro </i>and <i>in vivo</i>. Actin is a globular protein that forms linear polymeric microfilament in eukaryotic cells, and is involved in essential cellular functions such as muscle contraction, cell motility, cell adhesion, and cell shape. In neurons, this cytoskeletal protein is also involved in the formation of gap and synaptic junctions that are essential for intercellular communication responsible for processes such as nerve propagation and memory formation. We will discuss the examination of an actin bundling protein that can be modulated by small molecule inhibitors and agonists. Recent results will be presented to highlight the mechanistic details of how these molecules influence activity on actin bundling. We will also present results from <i>in vivo</i> and organotypic brain slice culture studies that highlight the effects of actin bundling modulation on synapse formation and memory and learning.