Urartu Seker1,Recep Ahan1,Cemile Ozcelik1,Ebuzer Kalyoncu1
Bilkent University1
Urartu Seker1,Recep Ahan1,Cemile Ozcelik1,Ebuzer Kalyoncu1
Bilkent University1
Many biological structures contain spatially organized materials produced by cells. Appearance of organized materials in distinct kingdoms indicates that organized materials provide advantages against natural selection. There are many examples of biological process which harvest the power of unique feature of spatially organized structures. Here, we developed recombinase enabled logic gates to synthesize organized materials in <i>E. coli</i>. The gates can utilize to record exposure of environmental signals and adjust to synthesize certain types of CsgA based fibrillous biofilm materials. Composition of fibrillous/cell biofilm material can be regulated by user-defined signals. We envision that recombinase based genetic circuits provide available route to the control composition and spatial organization of living materials.<br/><br/>The study was supported by TUBITAK Grant Numbers 114M163 and 216M127. U.O.S.S. thanks the TUBA-GEBIP Award. E.K. thanks TUBITAK BIDEB Graduate Fellowship.