Su-Hwan Kim1
Dong-A University1
Hydrogel system based on enzyme-mediated mild crosslinking reaction has been a promising approach in tissue engineering. Inspired by skin melanin synthesis and marine mussel adhesion, tyrosinase-mediated hydrogel crosslinking has been exploited as cell-friendly reactions and explicit reaction mechanisms. Hydrogel prepared by tyrosinase exhibits appealing properties as a dynamic scaffold for cell delivery and as a bioink for 3D bioprinting. Recapitulating the structure of the native extracellular matrix (ECM), innovative tyrosinase-mediated hydrogel crosslinking has now shifted to the field of translational medicine. Biomimetic hydrogel with in situ tyrosinase crosslinking can be efficiently and easily applicable to the disease model for therapeutic purposes. We demonstrate that the novel enzyme-based crosslinking hydrogel has a robust potential in tissue engineering and regenerative medicine.