Available on-demand - F.SM04.02.02
Polydopamine Interfaces for Integration of Photosynthetic Enzymes on Electrodes
Gianluca Farinola1,Gabriella Buscemi1,Roberta Ragni1,Francesco Milano2,Danilo Vona1,Massimo Trotta3
University degli Studi-Bari Aldo Moro1,CNR-ISPA2,CNR-IPCF3
The reaction center (RC) photoenzymefrom Rhodobacter sphaeroidesphotosynthetic bacterium is a membranespanning proteincapable to convert solar energy intocharge separated states with efficiency close to 100%.The photogenerated electron-hole pairs can beexploited to produce photocurrents and the suitability of RC for photoconversioonin electronic and electrochemical devices is well estabilished [1,2].
However, the electronic interface of RC with electrodes and the photoenzyme stability after integration in devices still require optimization to improve theefficiency of thephotocurrent production . Enzyme stability can be improved by encapsulationin soft structures, such as vescicles  and polymersomes .Electron transfer between RC and electrode surface can occur either directly or through electrochemical mediators. In the first case a suitable immobilization strategy is required . Although in the second case the RC can be dispersed in solution, higher photocurrents are produced if it is immobilized onto the electrode. To this aim,variousprotocols are reported in literature,and we have recently demonstrated thatmodification under mild temperature and pH condititons of RC with biomimetic and adhesive polymers, such as polydopamine,is a very promising method to improve the electrode/protein interface without altering the enzyme photoactivity .
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