Giuseppina Tommasini1,Susel Del Sol-Fernández1,Angela Tino2,Claudia Tortiglione2,Maria Moros1,3
Institute of Nanoscience and Materials of Aragon (INMA-CSIC)1,Institute of Applied Science and Intelligent System (ISASI-CNR)2,Centro de Investigación Biomédica en red en Bioingenieria, Biomateriales y Nanomedicina (CIBER-BBN)3
Giuseppina Tommasini1,Susel Del Sol-Fernández1,Angela Tino2,Claudia Tortiglione2,Maria Moros1,3
Institute of Nanoscience and Materials of Aragon (INMA-CSIC)1,Institute of Applied Science and Intelligent System (ISASI-CNR)2,Centro de Investigación Biomédica en red en Bioingenieria, Biomateriales y Nanomedicina (CIBER-BBN)3
Regenerative medicine is a pioneering field aimed at restoring and regenerating damaged tissues and organs through numerous strategies, including the use of new materials and de novo generated cells<sup>1-2</sup>. As a dynamic biological process, tissue regeneration requires a variety of intracellular and extracellular molecular mechanisms to facilitate cell division and regeneration. In recent years, multiple studies have demonstrated a central role of reactive oxygen species (ROS) in tissue regeneration, acting as signaling molecules that trigger the activation of molecular pathways involved with cell proliferation and differentiation<sup>3</sup>. Recently, regenerative medicine has found a surge of interest in magnetic nanoparticles, using their magnetomechanical and heating potential to activate different pathways. In this optics, a promising therapeutic approach could be the possibility of manipulating the ROS production to enhance tissue repair using mild magnetic hyperthermia (MH).<br/>Here, we present a novel approach for enhancing tissue regeneration in vivo through mild magnetothermal stimulation using manganese iron oxide nanoparticles (MnxFe3-xO4) with improved heating performance. We evaluated the biological effect of internalized MNPs subjected to an alternating magnetic field on the regeneration process that physiologically occurs in the freshwater invertebrate polyps Hydra vulgaris. To decipher the molecular mechanism underlying this process, we performed gene expression analysis of molecular markers normally modulated in ROS homeostasis in regenerated polyps after the MH stimulation. This innovative approach has not been applied before to improve tissue regeneration and thus could represent a breakthrough in tissue engineering.<br/><br/>1. Mao AS, Mooney DJ. Regenerative medicine: Current therapies and future directions. Proc Natl Acad Sci U S A. 2015 Nov 24;112(47):14452-9. doi: 10.1073/pnas.1508520112. PMID: 26598661; PMCID: PMC4664309.<br/><br/>2. Kara L. McKinley et al., Emerging frontiers in regenerative medicine. Science 380,796-798(2023). DOI:10.1126/science.add6492<br/><br/>3. Jing Zhou et al., Reactive oxygen species-sensitive materials: A promising strategy for regulating inflammation and favoring tissue regeneration, Smart Materials in Medicine, Volume 4, 2023, Pages 427-446.