TissueFab^® Ready-to-Use Bioinks for the Fabrication of Tissue Constructs

3D bioprinting is a powerful enabling tool for regenerative medicine and drug discovery. Despite promising advancements in 3D bioprinting technology, a need still remains for high quality commercially available ready-to-use bioinks to empower these studies. In response, we have developed a platform of bioinks, <i>TissueFab^®</i>, that are compatible with diverse widely studied cell types and bioprinting platforms. <i>TissueFab^®</i> is a family of ready-to-use bioinks based on natural proteins and polysaccharides and synthetic polymers with high batch-to-batch consistency. These bioinks are designed for optimal printability and mechanical properties for micro-extrusion based bioprinting which we verified on the most popular commercial bioprinters thus demonstrating the printer agnostic characteristic of the bioinks. The bioinks were designed to photochemically crosslink with a wide range of compatible wavelengths of light. Separately, moving past photo-crosslinking, we have developed a class of ionically crosslinkable bioinks, Facile Curable, which avoid the use of light and photoinitiators to form a stable construct while maintaining high cell viability. <i>TissueFab^®</i> bioinks were validated for high cell viability, proliferation and metabolic activity using C2C12 mouse myoblast cells, human mesenchymal stem cells (hMSCs) or human adult dermal fibroblasts (HDFa). For bone tissue engineering applications, we have shown that hMSCs bioprinted in <i>TissueFab^® - Bone bioinks </i>show an increase in osteogenic differentiation. Additionally, <i>TissueFab^®- Conductive bioinks</i> exhibit enhanced conductivity making them attractive for bioprinting electroactive tissues such as neural or muscular tissue. <i>TissueFab^®</i> bioinks provide a robust tissue-mimetic platform for 3D bioprinting applications with both high printability and cytocompatibility to aide in addressing drug testing and tissue engineering challenges.