Monitoring of 3D-Printed Airway Model Integrity with an Printed Organic Electrochemical Transistor

SARS-CoV-2 pandemic has drawn new attention to virus neutralization assay and neutralizing antibody quantification for drug testing preclinical testing and determining drug testing efficacy over time. Disruption of epithelial barriers is a key clinical turning point that differentiates patients who are likely to develop severe virus outcomes. However, convenient, fast and accurate barrier measurement remains a challenge. Organic electrochemical transistor(OECT) has shown a great potential particularly in capturing ionic signals, therefore extensively employed to probe local ion flux changes that are caused by paracellular flow of soluble molecules controlled by tight junctions. Here, we report inkjet printed PEDOT:PSS-based OECT for barrier measurement platform with inkjet printed 3D-printed airway model. We have developed a reliable printing process for fabricating OECT devices. Volumetric capacitance and transconductance of a device were significantly enhanced as the number of printed layered increased.Transient response in OECT could evaluate 3D-printed airway model. Finally, Influenza A virus infection was monitored in a 3D airway model with OECT. We could be detected barrier integrity with the advantages of a noninvasive process. Our platform show promise for use in vaccine evaluation.