Fabrication and Quantitative Performance Analysis of a High-Throughput CNT-DNA-Based Biosensor

The quest for highly efficient probes in the precise detection of single biomarkers has emerged as a compelling research field crucial for biological and biochemical studies. Over recent decades, this pursuit has been seen in not only small chemical probe molecules but also biomacromolecules such as enzymes, antibodies, and nucleic acids in the construction of biosensor platforms, enabling highly specific and efficient biomarker detection. Despite significant progress, there remains a pressing need to enhance biosensor performance, particularly for high-throughput clinical applications.<br/><br/>Among the tools contributing to single nucleotide detection, DNA-based biosensors stand out for their versatility. Their notable attributes, including good stability, high specificity, and excellent biocompatibility, make them valuable in both in vitro and in vivo applications. Here, we present a new DNA-modified CNT LoC sensor for small nucleic acid detection. Together with the probing platform, our novel biosensor platform excels in measuring the electrical signals of DNA with single-molecule precision while also enabling prolonged high-throughput measurements and the efficient screening of valuable data.<br/><br/>We anticipate that this platform will prove beneficial to individuals interested not only in biosensor technology but also in the broader realm of single-molecular detection.