Self-Organizing Peptides for Nanobioelectronics with 2D Materials

The physical properties of two-dimensional (2D) nanomaterials, such as graphene and 2D transition metal chalcogenides, have been extensively elucidated, revealing their potential for diverse applications. In the post-COVID era, the interface between biomolecules and 2D nanomaterials has gained significant attention due to their anticipated applications in biosensors. This presentation will explore recent advancements in controlling the biomolecule-nanomaterial interface on 2D surfaces using peptides that form ordered self-assembled structures.<br/>We focus on solid-binding peptides that form uniform monomolecular layers on 2D nanomaterial surfaces through self-organization [1-4]. The self-organization of these peptides is crucial as it allows for precise and consistent interface control, which is essential for the reliable performance of biosensors. These peptides can adapt their structure based on the electrochemical potential of the underlying 2D material [5] and modulate the electronic state of the 2D materials in response to changes in the solution's pH [6]. Additionally, the structure of the self-assembled organization can be altered by incorporating organic solvents into the solution [7]. These peptides also function as electrochemical catalysts [8], broadening the scope of interface control.<br/>A notable recent advancement is the development of graphene-based odor sensors using peptides that mimic the olfactory receptors of biological organisms. These sensors exhibit superior sensitivity and selectivity [9-11]. Specifically, a limonene-binding peptide was utilized as a bioprobe to detect the scent of lemon, successfully distinguishing the enantiomers of the limonene molecule with a remarkable 35-fold signal contrast. This significant signal contrast emphasizes the potential of these peptide-based sensors in achieving high sensitivity and specificity in odor detection. This presentation will detail these advancements and discuss the broader implications for controlling nanomaterial interfaces through self-organization of peptides.<br/><br/>Keywords: Self-organization, Peptide, Biosensor, Nano-Bio Interface<br/><br/>1. C. R. So, et.al., ACS Nano, 6 (2) 1648-1656 (2012).<br/>2. D. Khatayevich, et al., Small 10, 8 1505-1513 (2014).<br/>3. P. Li, et.al., ACS Applied Materials & Interfaces 11, 20670 (2019).<br/>4. Y. Hayamizu, et.al., Scientific Reports 6, 33778 (2016).<br/>5. T. Seki, T. Ihara, Y. Kanemitsu, and Y. Hayamizu, 2D Mater. 7, 034001 (2020).<br/>6. S. Tezuka, T. Seki, et.al. 2D Mater. 7, 024002 (2020)<br/>7. R. Ccorahua, et.al.<i> The Journal of Physical Chemistry B</i> 125.39 10893-10899 (2021).<br/>8. W. Luo, et al. <i>Nanoscale</i> 14.23 8326-8331 (2022).<br/>9. C. Homma, et.al., Biosensors and Bioelectronics, 224, 115047 (2023).<br/>10. T. Rungreungthanapol, et.al., Analytical Chemistry, 95(9), 4556--4563 (2023)<br/>11. Y. Yamazaki, et.al., ACS Appl. Mater. Interfaces 16, 18564–18573 (2024).