April 7 - 11, 2025
Seattle, Washington
Symposium Supporters
2025 MRS Spring Meeting & Exhibit
SB08.07.07

A Facile Approach to Prepare Proliposomes via Electrospinning

When and Where

Apr 11, 2025
11:00am - 11:15am
Summit, Level 3, Room 324

Presenter(s)

Co-Author(s)

Serdar Tort1,N. Basaran Mutlu Agardan1

Gazi University Faculty of Pharmacy1

Abstract

Serdar Tort1,N. Basaran Mutlu Agardan1

Gazi University Faculty of Pharmacy1
In recent years, electrospinning technique has become very popular in producing nanofibers. Electrospun nanofibers provide advantages in different areas such as filtration, textile, sensor, and biomedical. Various drug molecules could be loaded into nanofibers using different electrospinning methods. Drug-loaded nanofibers offer large surface area, high porosity, and increased drug solubility compared to conventional drug delivery systems. Liposomes facilitate the encapsulation of hydrophilic drugs within their core, while lipophilic drugs are incorporated into the lipid bilayers. Proliposomes are dry formulations consisting of a mixture of lipid components and the drug, which forms liposomes upon rehydration in the presence of an aqueous medium upon administration (1). Owing to its dry form, proliposomes provide many benefits compared to liposomes such as prolonged stability and the ability to rapidly convert into liposomes before application. The use of electrospinning method in producing proliposomes provides a one-step production of liposomes without extrusion or sonication processes. Proliposomal nanofibers were prepared with different polyvinylpyrrolidone (PVP), phosphatidylcholine and cholesterol ratios (2,3). PVP was used as an electrospinning polymer. Phosphatidylcholine provides the formation of lipid bilayer membrane, where cholesterol molecules provide stability and fluidity to the phospholipid membrane. Two different hydrophobic active ingredients were used as model drug molecules. According to DSC, FT-IR and XRD studies, drug molecules were encapsulated in amorphous form into nanofiber proliposomes. The liposomes occurred after the hydration of proliposomal nanofibers and their particle size and PDI values were found to be very low. TEM images showed that the self-assembled liposomes were obtained by hydration of nanofibers within seconds. Drug release from self-assembled liposomes changed with the polymer/lipid ratios. Production of proliposomal nanofibers via electrospinning could be a promising method for production of ready-to-use liposome formulations.

1. Yu, D. G., Branford-White, C., Williams, G. R., Bligh, S. A., White, K., Zhu, L. M., & Chatterton, N. P. (2011). Self-assembled liposomes from amphiphilic electrospun nanofibers. Soft Matter, 7(18), 8239-8247.

2. Tort, S., Öztürk, Z. C., Kaynak-Onurdağ, F., & Mutlu-Ağardan, N. B. (2024). Preparation and evaluation the effects of retinoic acid loaded proliposomal nanofibers on microbial biofilm inhibition. Pharmaceutical Development and Technology, 1–11.

3. Mutlu-Ağardan, N. B., & Tort, S. (2023). Cholesterol included self-assembled electrospun proliposomes as a feasible approach for drug delivery. Journal of Drug Delivery Science and Technology, 86, 104749.

Symposium Organizers

Andrew Steckl, University of Cincinnati
Luana Persano, Istituto Nanoscienze del Consiglio Nazionale delle Ricerche
Gregory Rutledge, Massachusetts Institute of Technology
Eyal Zussman, Technion

Symposium Support

Bronze
DOXA MICROFLUIDICS S.L.
Elmarco s.r.o.
Materic

Session Chairs

Urszula Stachewicz
Gareth Williams

In this Session