April 7 - 11, 2025
Seattle, Washington
Symposium Supporters
2025 MRS Spring Meeting & Exhibit
SB09.06.04

N-Acetyl-β-D-Glucosaminidase Activity Assay for Monitoring Insulin-Dependent Diabetes Using Ag-Porous Si SERS Substrate

When and Where

Apr 10, 2025
10:00am - 10:15am
Summit, Level 3, Room 333

Presenter(s)

Co-Author(s)

Giorgi Shtenberg1,Narsingh Nirala1

ARO Volcani Center1

Abstract

Giorgi Shtenberg1,Narsingh Nirala1

ARO Volcani Center1
Determination of urinary or serum N-acetyl-β-D-glucosaminidase (NAG) activity as a tissue damage indicator is widely used in diagnosis of various pathologies, including diabetic nephropathy. Early and rapid biomarker detection is an important element of medical diagnosis, facilitating prompt therapeutic decisions and prognosis evaluation. Herein, we present a modified sensing approach for a rapid and reliable NAG activity determination in complex media using surface-enhanced Raman spectroscopy (SERS). Porous silicon (PSi) Fabry-Pérot interferometers were redesigned as sensitive SERS platforms utilizing the vast inherent surface area for silver (Ag) nanoparticles embedment. Interaction of the porous nanostructures with specific NAG-enzymatic products produces an indicative spectral fingerprint proportional in magnitude to its concentration. The sensitivity of Ag-PSi SERS substrates was evaluated in complex matrices presenting sufficient limits of detection compared with other advanced assays and techniques (0.07, 0.47 and 0.50 mU mL-1 for urine, milk and plasma, respectively). The augmented optical performance revealed recovery values of 96-109%, indicating successful and selective NAG recognition in biological fluids. Finally, the potential applicability of the suggested prototype for real-life scenarios was evaluated in vivo, in a model of insulin-dependent diabetes induced in sheep. Overall, the robust data confirm the application of SERS analysis for early diagnosis of pathology and for evaluation of clinical responses to pharmacological treatments.

Keywords

nanoscale | surface enhanced Raman spectroscopy (SERS)

Symposium Organizers

Hendrik Heinz, University of Colorado at Boulder
Yuhei Hayamizu, Tokyo Inst of Technology
Sahika Inal, King Abdullah University of Science and Technology
Deniz Yucesoy, Izmir Institute of Technology

Session Chairs

Yuhei Hayamizu
Hendrik Heinz

In this Session