Additive Manufacturing of 3D Microchambers for Cell Culture

Two-photon polymerization (TPP) has been developed as a direct laser writing technique for the preparation of complex 3D structures with resolution beyond the diffraction limit. Its remarkable characteristics, such as precise 3D fabrication, sub-diffraction resolution, material flexibility, and mild processing conditions, have made it suitable for several applications in biosciences. One such application is the fabrication of microchamber scaffolds for cell culture, which are three-dimensional structures that provide mechanical support and a three-dimensional environment for cells growth and differentiation. Here, we report the fabrication of 3D square microchambers scaffold using TPP technique. Fabricated scaffolds are made up of microchannels and microchambers interconnected to create a porous structure that mimics the extracellular matrix (ECM) of natural tissues that can provide a suitable environment for cell growth and differentiation. These scaffolds can be used in tissue engineering and regenerative medicine applications.