April 22 - 26, 2024
Seattle, Washington
May 7 - 9, 2024 (Virtual)
Symposium Supporters
2024 MRS Spring Meeting
SB01.04.07

Understanding Structure and Stability for The Development of Novel Artificial Oxygen Carriers

When and Where

Apr 24, 2024
11:00am - 11:15am
Room 428, Level 4, Summit

Presenter(s)

Co-Author(s)

Martin Schroer1,Jan-Eric Sydow2,Fabian Nocke2,Ozan Karaman2,Katja Ferenz2,1

University of Duisburg-Essen1,University Hospital Essen / University of Duisburg-Essen2

Abstract

Martin Schroer1,Jan-Eric Sydow2,Fabian Nocke2,Ozan Karaman2,Katja Ferenz2,1

University of Duisburg-Essen1,University Hospital Essen / University of Duisburg-Essen2
Red blood cell concentrates (RBC) are an indispensable tool in various clinical scenarios; however, the available quantity is already highly limited, which will become an even more severe problem in the future. To overcome these peculiarities and limitations of RBCs, we developed albumin-derived perfluorocarbon-based artificial oxygen carriers (A-AOCs), that are comprised of a perfluorodecalin (PFD) nano-emulsion core and an albumin shell, and have already successfully supplied oxygen in extra-corporally perfused hearts and various animal models <b>[1]</b>.<br/><br/>The structure and stability of A-AOCs is sensitive to changes of the carrier solution, so we need to learn more about the structure and structural changes on the nanoscale. Conventional characterization techniques lack the spatial resolution (<i>e.g.</i> light scattering) or need dried and consequently non-physiological samples (<i>e.g.</i> SEM, TEM), and thus yield limited information on A-AOC structure in solution. We therefore use X-ray scattering techniques to study A-AOCs, which do not have such limitations and allow also to study dense, opaque suspensions.<br/><br/>In this contribution, we present recent results of X-ray scattering measurements on A-AOCs covering a wide, clinical-relevant concentration and temperature range. We investigate A-AOCs from different synthesis approaches, using ultra-sound <b>[2]</b> and microfluidizing techniques, which reveal different sizes and stabilities. Based on these findings, it is now possible to optimize the synthesis parameters, in order to yield stable A-AOCs of desired size and dispersity.<br/><br/>This study demonstrates the huge potential of X-ray scattering for the study of pharmaceutical and medical-relevant samples <b>[3]</b>, with direct implications for the development of new formulations.<br/><br/><br/><b>References:</b><br/><b>[1]</b> J. Jägers, A. Wrobeln, K. B. Ferenz, <i>Pflugers. Arch.</i> <b>473</b>, 139 (2021).<br/><b>[2]</b> A. Wrobeln, J. Laudien, C. Gross-Heitfeld, J. Linders, C. Mayer, B. Wilde, T. Knoll, D. Naglav, M. Kirsch, K. B. Ferenz, <i>Eur. J. Pharm. Biopharm. </i><b>115</b>, 52 (2017).<br/><b>[3]</b> S. S. Nogueira, A. Schlegel, K. Maxeiner, B. Weber, M. Barz, M. A. Schroer, C. E. Blanchet, D. I. Svergun, S. Ramishetti, D. Peer, P. Langguth, U. Sahin, H. Haas, <i>ACS Appl. Nano Mater.</i> <b>3</b>, 1063 (2020).

Keywords

biomaterial

Symposium Organizers

Weibo Cai, University of Wisconsin--Madison
Bella Manshian, KU Leuven
Dalong Ni, Shanghai Jiao Tong University.
Ruirui Qiao, The University of Queensland

Symposium Support

Bronze
EXODUS BIO
JINAN NANOMEDICINE (HONG KONG) CO., LIMITED
Journal of Nanobiotechnology
KeAi-Bioactive Materials
POP Biotechnologies
Portrai Inc.
Promega Corporation
RAYSOLUTION Healthcare Co., Ltd
Shandong Madic Technology Co., Ltd.
United Well Technologies (China) Limited

Session Chairs

Ruirui Qiao
Hao Song

In this Session