December 1 - 6, 2024
Boston, Massachusetts
Symposium Supporters
2024 MRS Fall Meeting & Exhibit
CH05.16.02

Imaging the Smallest Living Things with Nanometer-Resolution Without Compromising Viability

When and Where

Dec 5, 2024
1:45pm - 2:00pm
Sheraton, Third Floor, Fairfax B

Presenter(s)

Co-Author(s)

Ashutosh Kumar1,Nicolas Perry1,Apurba Paul1,Mehmet Ozdogan1,Gregory Timp1

University of Notre Dame1

Abstract

Ashutosh Kumar1,Nicolas Perry1,Apurba Paul1,Mehmet Ozdogan1,Gregory Timp1

University of Notre Dame1
This work represents a first step towards the illumination of the biological mechanisms underpinning live cell physiology with nanometer resolution. Using low-energy (30 keV), low-dose, probe-corrected, integrated differential phase-contrast scanning transmission electron microscopy (iDPC-STEM) in conjunction with a liquid flow cell, two genetically engineered Mycoplasma species, M. mobile and M. pneumoniae, which are among the smallest, simplest, self-replicating bacteria, were scrutinized with nanometer-resolution without compromising cell viability. The viability was scored at a lethal dose to 50% of the population at LD50 > 3600 e-/nm2 at a beam energy of 30 keV by expression of an inducible fluorescent reporter following exposure to the electron beam in genetically engineered strains of the bacteria, which is in stark contrast with the LD50 < 56 e-/nm2 observed at 300 keV. The higher LD50 at a beam energy of 30 keV opened a wide window for high-resolution imaging of cell physiology. Within this window, the mechanisms for gliding motility in Mycoplasma, which are essential to infection and mediate attachment to a host, were elucidated with < 3 nm resolution.

Keywords

biological | scanning transmission electron microscopy (STEM) | transmission electron microscopy (TEM)

Symposium Organizers

Miaofang Chi, Oak Ridge National Laboratory
Ryo Ishikawa, The University of Tokyo
Robert Klie, University of Illinois at Chicago
Quentin Ramasse, SuperSTEM Laboratory

Symposium Support

Bronze
EKSPLA 
Protochips
Thermo Fisher Scientific, Inc.

Session Chairs

Ryo Ishikawa
Quentin Ramasse

In this Session