December 1 - 6, 2024
Boston, Massachusetts
Symposium Supporters
2024 MRS Fall Meeting & Exhibit

Hemagglutination Assay via Optical Density Characterization in 3D Microtrap Chips

When and Where

Dec 4, 2024
8:00pm - 10:00pm
Hynes, Level 1, Hall A

Presenter(s)

Co-Author(s)

Dong-Gyu Jeon1,Sung-Wook Nam1

Kyungpook National University1

Abstract

Dong-Gyu Jeon1,Sung-Wook Nam1

Kyungpook National University1
Hemagglutination assay has been used for blood typing and detecting viruses, thus applicable for the diagnosis of infectious diseases, including COVID-19. Therefore, the development of microfluidic devices for fast detection of hemagglutination is on-demand for point-of-care diagnosis. Here, we present a way to detect hemagglutination in 3D microfluidic devices via optical absorbance (optical density, OD) characterization. 3D printing is a powerful way to build microfluidic structures for diagnostic devices. However, mixing liquid in microfluidic chips is difficult due to laminar flow, which hampers practical applications such as antigen-antibody mixing. To overcome the issue, we fabricated 3D microfluidic chips with embedded microchannel and microwell structures to induce hemagglutination between red blood cells (RBCs) and antibodies. We named it a 3D microtrap chip. We also established an automated measurement system which is an integral part of diagnostic devices. To do this, we developed a novel way to identify RBC agglutination and non-agglutination via the OD difference. By adapting a 3D-printed aperture to the microtrap chip, we obtained a pure absorbance signal from the microchannels by eliminating the background brightness of the microtrap chip. By investigating the underlying optical physics, we provide a 3D device platform for detecting hemagglutination.

Keywords

metrology | self-assembly

Symposium Organizers

Elizabeth Cosgriff-Hernandez, The University of Texas at Austin
Reza Foudazi, The University of Oklahoma
Markus Muellner, The University of Sydney
Christine Selhuber-Unkel, Heidelberg University

Symposium Support

Bronze
Nature Materials

Session Chairs

Elizabeth Cosgriff-Hernandez
Reza Foudazi
Markus Muellner
Christine Selhuber-Unkel

In this Session