In Vitro Validation of PEDOT:PSS 3D Printed Conductive Hydrogels as Promotors of Angiogenesis and Wound-Healing in Diabetic Foot Ulcers (DFU)

Diabetic foot ulcers (DFUs) represent a significant healthcare challenge worldwide for patients with diabetes Mellitus. These wounds are associated with poor glycemic control, vascular disease, and underlying neuropathies. Alongside impaired wound healing and bacterial infection biofilms, DFUs often become a chronic disease that can lead to severe complications such as osteomyelitis and lower limb amputations. In this research project, we propose an innovative therapeutic strategy, applying polymeric conductive 3D-printed hydrogels to promote angiogenesis and improve wound healing in DFUs. These hydrogels, composed of biocompatible polymers, offer a combination of electrical conductivity and mechanical properties suitable for wound healing applications. The conductive properties of the hydrogels facilitate electrical stimulation at the wound site, promoting cell proliferation, angiogenesis, tissue regeneration, and inhibiting bacterial growth. The antimicrobial activity of the hydrogel is of high relevance, as progressed polymicrobial biofilms become drug-resistant and can lead to treatment failure. Additionally, the 3D printing technology allows for precise customization of the hydrogel scaffolds, ensuring optimal fit and coverage of the ulcerated area.<br/><br/>At this stage, the project aims to investigate the efficacy of conductive 3D-printed hydrogels in promoting wound healing and preventing complications associated with DFUs through in vitro experiments in hyperglycemia conditions. Additionally, biocompatibility and long-term safety assessments will be conducted to evaluate the feasibility of clinical translation. The outcomes of this research can potentially translate into improved therapeutic approaches in the management of DFUs, offering a promising addition to the current standard treatment and improving the quality of life for diabetic patients.<br/><br/>This research project has been supported by the Challenge Research Funding Program of Tecnologico de Monterrey.