High-Brightness Fluorophores (HBFs) for Rare Antigen Detection

Cancer immunotherapy has revolutionized in recent years thanks to cutting-edge tools that have empowered researchers to understand adaptive immune systems better. Flow cytometry, for example, has become indispensable for immunological research and has evolved from a single to 50-color parameters. This multiplexing capability in flow cytometry is decisive for understanding complex tumor samples. However, there is a delicate balance between the quality and quantity of data. Spillover and auto-fluorescence have continued to limit multi-color measurement. Brighter dyes or fluorophores with high resolution are essential to ensure high-quality data.<br/>Introducing a breakthrough in our field, we present a new series of high-brightness fluorophores (HBFs) that promise to revolutionize rare antigen detection. Our NovoLux646, for instance, when conjugated with anti-human CD127, delivers a staining index (SI) of 107, a staggering 20X higher than that from Anti-CD 127-APC. NovoLux646 also outshines AlexaFluor647 in brightness. We also evaluated our violet NovoLux420 for CD 127 detection, achieving an SI as high as 222 from Anti-CD 127- NovoLux420, 16X brighter than cFluor420. These results hint at the potential of our HBFs to enable the detection of many more rare antigens, sparking a new era in immunological research. Details of our findings will be discussed at the conference.<br/><br/>We acknowledge the support from the National Science Foundation (Award number 1738466).