2:30 PM - *SB02.08.05
3D Bioprinting—What Cells Find Attractive in a Gel
Gordon Wallace1
University of Wollongong1
Show Abstract
3D Bioprinting enables the fabrication of structures with living cells, biomaterials and other active ingredients (e.g. drugs and/or growth factors) strategically arranged in three dimensions. The biomaterials used are traditionally hydrogels since these provide adequate mechanical support to ensure structure integrity along with a cytocompatible environment.
The engagement of cells with the biomaterial (gel or the precursors) during and after printing determines the success or otherwise of any 3D Bioprinting strategy.
The interaction of gel or the precursors and cells determines the ability to retain viable suspensions in bioink reservoirs.
The rheological properties of the gels used determines how they behave and how they might protect cells during printing.
The modulus and porosity of gels determines the capacity of cells to interact with each other and the immediate environment.
Using several 3D bioprinting case studies aimed at meeting clinical challenges we will explore the critical properties of gels for such applications.
The clinical challenges include:
- cartilage regeneration in the knee (1,2)
- islet cell transplant ion to treat diabetes (3,4)
- skin regeneration (5,6)
With a view to developing protocols that are implementable in the clinic we will also review some of the additional requirements such gels must meet. These include the availability of a reliable and reproducible source material and compatibility with sterilisation methods traditionally used.
The cell - gel engagement is short lived and the temporal dimension is critical to a successful relationship. The role of the gel as a function of cell and tissue development time will be discussed.
References
(1) Duchi, S., Francis, S., O’Connell, C.D., Aguilar, L.M.C., Doyle, S., Yue, Z., Wallace, G.G., Choong, P.F., Di Bella, C. FLASH: Fluorescently LAbelled Sensitive Hydrogel to monitor bioscaffolds degradation during neocartilage generation, Biomaterials 2021, 264, 120383.
(2) O’Connell, C.D., Di Bella, C., Thompson, F., Augustine, C., Beirne, S., Cornock, R., Richards, C.J., Chung, J., Gambhir, S., Yue, Z., Bourke, J., Zhang, B., Taylor, A., Quigley, A., Kapsa, R., Choong, P., Wallace, G.G. Development of the Biopen: A handheld device for surgical printing of adipose stem cells at a chondral wound site, Biofabrication 2016 8, 015019.
(3) Kim, J., Hope, C.M., Gantumur, N., Perkins, G.B., Stead, S.O., Yue, Z., Liu, X., Asua, A.U., Kette, F.D., Penko, D., Drogemuller, C.J., Carroll, R.P., Barry, S.C., Wallace, G.G., Coates, P.T. Encapsulation of Human Natural and Induced Regulatory T-Cells in IL-2 and CCL1 Supplemented Alginate-GelMA Hydrogel for 3D Bioprinting, Advanced Functional Materials 2020, 30 (15), 2000544.
(4) Liu, X., Carter, S.-S.D., Renes, M.J., Kim, J., Rojas-Canales, D.M., Penko, D., Angus, C., Beirne, S., Drogemuller, C.J., Yue, Z., Coates, P.T., Wallace, G.G. Development of a co-axial 3D printing platform for biofabrication of implantable islet-containing constructs, Advanced Healthcare Materials 2019, 8 (7), 1801181.
(5) Zhou, Y., Kang, L., Yue, Z., Liu, X., Wallace, G.G. Composite Tissue Adhesive Containing Catechol-Modified Hyaluronic Acid and Poly-L-lysine, ACS Applied Bio Materials 2020, 3 (1), 628-638.
(6) Daikuara, L.Y., Yue, Z., Skropeta, D., Wallace, G.G. In vitro characterisation of 3D printed platelet lysate-based bioink for potential application in skin tissue engineering, Acta Biomaterialia 2021, 123, 286-297.